Fig. 2
TFDM protected U87 cells against H2O2-induced apoptosis. a Annexin V-FITC/PI double staining was further used to investigate the anti-apoptotic role of TFDM by flow cytometry. b TFDM decreased the mean fluorescence intensity of Annexin V-FITC/PI in U87 cells after H2O2-induced injury. c-e Representative mRNA levels of Caspase-12, Caspase-9 and Bax, as measured by qPCR, are shown. f-j Apoptosis-related protein levels in H2O2-treated U87 cells. The data are presented as the mean ± SD, n = 3. #p < 0.05 vs. control; *p < 0.05, **p < 0.01, ***p < 0.001 vs. H2O2