Fig. 5

The effect of CF on the expression of osteoclastogenesis-related genes. RAW 264.7 cells were treated with RANKL (100 ng/ml) and CF (1, 10, 100 μg/ml) for 4 days. A RANK, TRAP, CTK, OSCAR, ATP6v0d2 and CA2 mRNA expression levels were detected using RT-PCR. a The mRNA expression was normalized to GAPDH. B CTK and MMP-9 protein expression levels were detected using western blot. b Protein expression was normalized to actin. Data represent the means ± SEM of three independent experiments. ^##P < 0.01, ^#P < 0.05 compared with normal and ^**P < 0.01, ^*P < 0.05 compared with control; RANK, receptor activator nuclear factor-κB; TRAP, tartrate-resistant acid phosphatase; CTK, cathepsin K; OSCAR, osteoclast-associated receptor; ATP6v0d2, ATPase H+ transporting V0 Subunit D2; CA2, Carbonic anhydrase II; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MMP-9, Matrix metallopeptidase 9; N, nontreated; C, RANKL-treated control