Fig. 6

Rhy mitigates asthma through inhibiting JAK2/STAT3 signaling pathway. a After treatment with TGF-β and Rhy or WP-1066, CCK-8 assay was performed to assess the proliferation of ASMCs. b-d The level of IL-6 and activities of SOD and CAT were detected. e-h The levels of LC3, beclin-1, ATG5 and p62 were detected by western blot. (i) MDC staining was performed to assess autophagy in ASMCs. Bar = 100 μm. The results were presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Rhy, rhynchophylline; JAK2, janus kinase 2; STAT3, signal transducer and activator of transcription 3; ASMC, airway smooth muscle cell; IL, interleukin; SOD, superoxide dismutase; CAT, catalase; LC3, microtubule associated protein 1 light chain 3; ATG5, autophagy related 5; SD, standard deviation