Table 1 Primer pairs used for real-time PCR analysis of target genes
Gene name | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
β-actin (rat) | CAGCTGAGAGGGAAATCGTG | CGTTGCCAATAGTGATGACC |
α-SMA (rat) | CTACATGCGTCTGGACTTGG | CCAGGGAAGAAGAGGAAGCA |
JAK2 (rat) | ATGTCAACCAACGTCCCTCT | GAGCTTTGCTCTGGTTCTGG |
STAT3 (rat) | GCTGGAACGGCATCTTCAGG | CTGTCTGGTCACAGACTGGT |
β-actin (mouse) | TATAAAACCCGGCGGCGCA | TCATCCATGGCGAACTGGTG |
α-SMA (mouse) | GTCCCTCTATGCCTCTGGAC | AAGGAATAGCCACGCTCAGT |
FSP-1 (mouse) | AGCTGCATTCCAGAAGGTGA | ATGCAGGACAGGAAGACACA |
Col I (mouse) | TGGAAACCCGAGGTATGCTT | CATTGCATTGCACGTCATCG |
Col III (mouse) | ACTGGTGAACGTGGCTCTAA | AACCTGGAGGACCTGGATTG |
FN (mouse) | TCACCTACGGAGAGACAGGA | TGTTGTTGATGGTGGCTGTG |
STAT3 (mouse) | TGGTGTCTCCACTTGTCTACCTCTAC | GTGTCACACAGATGAACTTGGTCTT |
JAK2 (mouse) | GGAATGGCCTGCCTTACAATG | TGGCTCTATCTGCTTCACAGAAT |
TGF-β (mouse) | TTGCTTCAGCTCCACAGAGA | CAGAAGTTGGCATGGTAGCC |
SOCS1 (mouse) | CCCTCTTAACCCGGTACTCC | CTCCCACGTGGTTCCAGAAA |
SOCS3 (mouse) | GCAGGAGAGCGGATTCTACT | TGGATGCGTAGGTTCTTGGT |