Fig. 3

GM reduced microglial activation in brain tissue of mice with TBI. a, b The mRNA expressions of microglial activation markers CD16 and CD11b in the brain tissue of mice in each group were detected by qRT-PCR assay. c Western blot assay was used to detect the expressions of microglia activation markers CD16 and CD11b in the brain tissue of mice in each group. Sham: Control group; CCI: TBI model group; CCI + GM 5 mg/kg: CCI mice treated with 5 mg/kg GM group; CCI + GM 10 mg/kg: CCI mice treated with 10 mg/kg GM group; CCI + GM 20 mg/kg: CCI mice treated with 20 mg/kg GM group;* P < 0.05, ** P < 0.01 and *** P < 0.001 versus the sham group; # P < 0.05, ## P < 0.01 and ### P < 0.001 versus the CCI group