Table 1 Primers sequences used for RT-PCR analyses
Name | 5→sequence→3 | Product size | NCBI Reference Sequences(Ref Seq) | |
|---|---|---|---|---|
GAPDH | Sense | CCAGAACATCATCCCTGCCT | 185 | NM_00125679 |
Antisense | CCTGCTTCACCACCTTCTTG | |||
COL2A1 | Sense | TCCACGGAAGGCTCCCAGAA | 141 | NM_001844.5 |
Antisense | CCTGCTATTGCCCTCTGCCC | |||
Aggrecan | Sense | CCTCTGGACAACCAGGTATTAG | 97 | NM_001135 |
Antisense | CCAGATGTTTCTCCACTCAGAT | |||
SOX9 | Sense | GAGCTGAGCAGCGACGTCAT | 130 | NM_000346.4 |
Antisense | CGTAGCTGCCCGTGTAGGTG | |||
KRT19 | Sense | GGAAGACACACTGGCAGAAA | 112 | NM_002276.5 |
Antisense | CTCACTATCAGCTCGCACATC | |||
PAX1 | Sense | CCGCTCGCTATGGAGCAGAC | 204 | NM_001257096.1 |
Antisense | GGAGCCGGTCTCGTTGTAGC |