Fig. 7
Effects of SCP-0.5 g and SCP-1 g treatments on NKCC1 and AQP4 expression in the penumbra of the cerebral cortex. Representative images show a NKCC1 and b AQP4 expression in the penumbra of the cerebral cortex in the Sham, Model, SCP-0.25 g, SCP-0.5 g, and SCP-1 g groups 3 d after reperfusion. c The dotted line square in a TTC-stained coronal brain section reveals the region in which the immunopositive cells are counted. CP, cortical penumbra. Dotted line square = 1 mm2. The bar graphs display the numbers of d NKCC1- and e AQP4-positive cells in the penumbra of the cerebral cortex among the experimental groups. *P < 0.05 compared with the Sham group; #P < 0.05 compared with the Model group. Arrows in (a) and (b) point to NKCC1- and AQP4-positive cells, respectively. Scale bars = 10 μm and 2 mm for (b) and (c), respectively