Fig. 2

QIG induced caspase-dependent apoptosis in CRC cells. a After treated with 0.1 mg/ml, 0.3 mg/ml and 0.5 mg/ml of QIG for 24 h, HT-29 and CT-26 cells were stained with Annexin V/PI and the apoptosis was analyzed by flow cytometry. b-c HT-29 cells were dose-dependently treated with QIG, then the protein levels of PARP, cleaved-PARP, cleaved-caspase 3, cleaved-caspase 9, Bax, Bcl-2, β-Tubulin and GAPDH were detected by western blotting and quantified by Image J software. d The expression of Cyto-c in mitochondrial and cytoplasm were detected by western blotting and quantified by Image J software