Table 2 Primer and siRNA sequences
From: Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene
Gene | Primer sequence | Amplified fragment size (bp) | Location |
|---|---|---|---|
Cathepsin B | Upstream primer: 5′-TACAATTCCTACAGCGTCTCC-3′ Upstream primer: 5′-GTGCCATTCTCCACTCCC-3′ | 194 | 679–872 |
Caspase-3 | Upstream primer: 5′-CATGGAAGCGAATCAATGGACT-3′ Upstream primer: 5′-CTGTACCAGACCGAGATGTCA-3′ | 139 | 64–202 |
GAPDH | Upstream primer: 5′-AATCCCATCACCATCTTC-3′ Upstream primer: 5′-AGGCTGTTGTCATACTTC-3′ | 218 | 436–653 |
siRNA-NC | 5′-GUACGCCAAAAGUUAAACC-3′ |  |  |
siRNA1 | 5′-CCUGUCGGAUGAGCUGGUCAACUAU-3′ |  |  |
siRNA2 | 5′-CCAGUACCUCCAAGCAAGUAGCUUU-3′ |  |  |
siRNA3 | 5′-UGCAUCUAUCGAGUUUGCAAUGUCA-3′ |  |  |