Fig. 5

JPYW induced BGC823/5-Fu apoptosis in vivo. a. Comparing the tumor volumes of mice, the growth inhibition of the JPYW-high group was obvious and the JPYW+ 5-Fu group showed the most obvious inhibitive effect of tumor growth, while there was no significant difference between the JPYW-low group and the control group; b. Tunel apoptosis detection showed that the JPYW-high group and JPYW+ 5-Fu group had dramatically increased numbers of TUNEL-positive cells. (Magnification 200×); c. The Tunel results showed that the apoptosis rates of the JPYW-high group and JPYW+ 5-Fu group were significantly increased compared with the control group, and the JPYW+ 5-Fu group showed higher apoptosis rate compared with JPYW-high group (P < 0.05); d. In the JPYW-low group and JPYW+ 5-Fu group, the expression of AKT mRNA was significantly decreased, while in the JPYW-high group and 5-Fu was significantly increased