Fig. 3
EAB extract and annonacin display dose dependent impact on cell viability of DU-145 cells. Antiproliferative activity of (a) annonacin, (b) ethyl acetate extract of A. muricata bark (EAB), (c) docetaxel and (d) docetaxel in combination with 100 μg/ml EAB extract against DU-145 prostate cancer cells determined by MTT assay after 72 h incubation with varying concentrations of each sample. Results are means ± SEM of triplicates in three independent experiments and the percentage of cell viability was calculated as a percentage of solvent control (1% DMSO). A dose dependent decrease in cell viability was observed with all test samples and combining docetaxel with the EAB extract reduced the observed IC50