Xanthine oxidase inhibitory activity of a new isocoumarin obtained from Marantodes pumilum var. pumila leaves

Table 2 IC₅₀ values of xanthine oxidase inhibition of selected extracts of Marantodes pumilum varieties and allopurinol

Species	Plant part	Crude extracts	Concentration (μg/mL)	Percentage of xanthine oxidase inhibition (%)	IC₅₀ value of xanthine oxidase inhibition (μg/mL)^a
M. pumilum var. alata	Leaves	DCM	25	28.08 ± 0.18	310.9 ± 8.25
			50	15.94 ± 0.00
			100	3.99 ± 0.00
			200	20.49 ± 0.22
			400	68.21 ± 2.50
M. pumilum var. pumila	Leaves	DCM	25	15.34 ± 0.25	161.6 ± 7.35
			50	14.31 ± 0.89
			100	23.85 ± 0.05
			200	65.28 ± 1.81
			400	85.77 ± 0.70
		MeOH	25	4.94 ± 0.29	175.1 ± 0.20
			50	9.86 ± 1.51
			100	20.08 ± 0.05
			200	60.73 ± 0.72
			400	80.97 ± 0.72
M. pumilum var. lanceolata	Leaves	DCM	25	26.90 ± 3.09	233.1 ± 19.85
			50	13.60 ± 2.93
			100	28.89 ± 0.87
			200	35.59 ± 1.40
			400	74.33 ± 4.33
		MeOH	25	19.15 ± 0.04	185.3 ± 2.50
			50	11.25 ± 1.21
			100	37.98 ± 0.88
			200	53.37 ± 0.37
			400	67.52 ± 0.35
Allopurinol (positive control)			0.0064	4.12 ± 0.00	0.24 ± 0.00
			0.032	17.81 ± 0.00
			0.16	29.47 ± 0.00
			0.8	93.66 ± 0.00
			4	98.86 ± 0.00
			20	99.01 ± 0.00
			100	99.82 ± 0.00

Data are presented as mean ± SEM. of three replicates (n = 3)
^aIC₅₀ values were obtained based on the percentage of xanthine oxidase inhibition of extracts and allopurinol at different concentrations using the Graphpad Prism 5 software (La Jolla, CA, USA)

ISSN: 2662-7671