Fig. 6

The effect of OIE on glucose metabolism uptake in 3 T3-L1 at 24 h; 48 h; and 12 days. The cells were incubated with 2-NBDG fluorescence dye for 60 min at different periods. Labeled cells were collected and analyzed using a flow cytometer with excitation 488 nm. Means ± SD value (n = 9) of three independent experiments is shown. ND: non-differentiated cells (pre-adipocytes); D: differentiated cells with 0.1% DMSO (untreated-adipocytes); D + OIE(200): differentiated cells with OIE at 200 μg/mL (OIE-treated adipocytes). Differences among groups were determined by one-way ANOVA followed by Tukey’s Post-hoc test; the different superscript alphabets are significantly different from each other (P < 0.05). The differences of glucose uptake between D + OIE during 24 h versus 12 days were analyzed by the Paired t-test, * P < 0.05