Fig. 5

The effect of OIE on GLUT4 expression in the 3 T3-L1 cell. 3 T3-L1 pre-adipocytes, adipocytes, and OIE treated-cells (200 μg/mL) were collected at 24 h and day 12. The cells were incubated with anti-GLUT4 antibody (primary antibody) and mouse IgGk (secondary antibody). Then, the cells were double-stained with DAPI (nucleus stains). a The pictures were taken at 24 h, representing cellular and nuclear morphology of the cells, Nuclei (blue), GLUT4 (red), and co-immunostaining (overlay all channels). b The pictures were taken on day 12, representing cellular and nuclear morphology of the cells, Nuclei (blue), GLUT4 (red), and co-immunostaining (overlay all channels). Scale bar; 50 μm. Two independent experiments (each run triplicate) were performed. ND: non-differentiated cells (pre-adipocytes); D: differentiated cells with 0.1% DMSO (untreated-adipocytes); D + OIE(200): differentiated cells with OIE at 200 μg/mL (OIE-treated adipocytes)