Fig. 4

The effect of OIE on glucose metabolism in 3 T3-L1 cells. a Western blot analysis displays the expression of PY20 and GLUT4 in cells treated with 200 μg/mL OIE at 24 h and 12 days. The total lysates of 3 T3-L1 cells were resolved on Mini-PROTEAN TGX gels followed by transfer to a PVDF membrane, probed with the anti-phosphotyrosine antibody, anti-glucose transporter 4 (primary antibody), and a secondary antibody. The protein was visualized using chemidoc with the ECL detection kit. b The densitometry analysis of Western blot bands was normalized against β-actin. Means ± SD value (n = 9) of three independent experiments is presented. PY20(H) = protein tyrosine phosphorylation with 181 or 164 kDa; PY20(L) = protein tyrosine phosphorylation with 91 kDa; ND: non-differentiated cells (pre-adipocytes); D: differentiated cells with 0.1% DMSO (untreated-adipocytes); D + OIE(200): differentiated cells with OIE at 200 μg/mL (OIE-treated adipocytes). Differences among groups were determined by one-way ANOVA followed by Tukey’s Post-hoc test, and the different superscript alphabets are significantly different from each other (P < 0.05). The full-length of the blots are presented in Supplementary Figure 2