Fig. 10
From: An insight into anti-adipogenic properties of an Oroxylum indicum (L.) Kurz extract
The effect of OIE on mitochondrial mass. On day 12, the cells were incubated with 200 nM of MitoTracker dyes for 45 min. Labeled cells were transferred to the Shandon cytofunnel chamber and mounted with the mounting media. Then, the cells were visualized by laser scanning microscopy (excitation 488 nm). Mitochondria labeled with MitoTracker dyes showed in green color, scale bar, 20 μm, and 5 μm. Two independent experiments (each run triplicate) were performed. ND: non-differentiated cells (pre-adipocytes); ND + OIE(200): non-differentiated cells with OIE at 200 μg/mL (OIE-treated pre-adipocytes); D: differentiated cells with 0.1% DMSO (untreated-adipocytes); D + OIE(200): differentiated cells with OIE at 200 μg/mL (OIE-treated adipocytes)