Fig. 3

Mitochondrial depolarisation of MC38 cells stained with JC-1 is shown by an increased ratio of the green/red fluorescence intensity measured by FACS analysis after 24 h-treatment with BAE at different ANC concentrations: (a) control; (b) positive; (c) 100 μM ANC; (d) 200 μM ANC; (e) 500 μM ANC; (f) 1000 μM ANC; (g) 500 μM BAE-L929 cells. Data are displayed as the mean ± S.D. of three independent experiments. *p < 0.05, ** p < 0.01, *** p < 0.001 respect to control cells by ANOVA