Fig. 1

Fat accumulation on the liver histopathology. a After embedding and sectioning the fixed liver tissue of all mice, hematoxylin and eosin (H&E) and Masson’s trichrome (M&T) stained liver sections are shown at 400x magnification. Frozen sections of liver tissue were stained with Oil Red O to detect intracellular lipid droplets after fixation. Three to five mice per group were used for the preparation of the tissue sections, and H&E, M&T and Oil Red O staining was analyzed in duplicate in each liver tissue. b After exposure to EMfC (50 mg/kg) for 12 weeks, the levels of PPAR-γ, C/EBPα, FAS, aP2 and Klf2 mRNA in the total transcripts of liver were measured by qRT-PCR analyses using their specific primers. The relative levels of the PPAR, C/EBPα, FAS, aP2 and Klf2 mRNA were calculated based on the intensity of β-actin as an endogenous control. Three to five mice per group were used in the preparation of the total RNAs, and qRT-PCR analyses was assayed in duplicate for each sample. Data represents the mean ± SD. Asterisk symbol (*) indicates significance P < 0.05 as compared to the No treated group. Sharp symbol (#) indicates significant P < 0.05 compared to the HFD + Vehicle treated group