Fig. 2From: Genistein contributes to cell cycle progression and regulates oxidative stress in primary culture of osteoblasts along with osteoclasts attenuationEffect of GS on osteoblasts differentiation and calcification (a) Photomicrographs show ALP stain in osteoblasts treated with increasing concentrations 1, 5 and 10 μM of GS at 48 h (b) Quantitative data of ALP level are presented in the form of ALP activity relative to control (c) Photomicrographs represent the increased formation of mineralized nodules of osteoblasts treated with increasing concentrations 1, 5 and 10 μM of GS at 21 days. Scale bars = 0.1 mm (d) Quantitative data of Alizarin Red-S extraction are expressed in the form of percent calcification. Data are represented as mean ± SEM of three independent experiments. *P < 0.05 as compared with controlBack to article page