Fig. 4
Reactive oxygen species (ROS) generation in response to coffee honey. The ROS levels of the control larvae (w; UAS-GFPIR/+;elav-GAL4/+), dUbqn knockdown larvae (w; UAS-dUbqnIR107–494/+;elav-GAL4) without treatment and with coffee honey treatment were measured using fluorescence emitted by dichlorofluorescein (DCF). Quantification results represent mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. (n = 3 of 20 larvae per group)