Table 1 Primers Sequences for qRT-PCR and MSP used in this study
From: Indirubin inhibits Wnt/β-catenin signal pathway via promoter demethylation of WIF-1
Inolucrin | F-TCAATACCCATCAGGAGCAAATG |
|---|---|
R-GAGCTCGACAGGCACCTTCT | |
TGase1 | F-TCTTCAAGAACCCCCTTCCC |
R-TCTGTAACCCAGAGCCCTTCGA | |
Keratin17 | F-GAGATTGCCACCTACCGC |
R-TGCCATCCTGGACCTGTT | |
Loricrin | F-TCATGATGCTACCCGAGGTTTG |
R-CAGAACTAGATGCAGCCGGAGA | |
Filaggrin | F-CTCAGCACAAGGAAGACAGG |
R- TTGTGTTCTGGTGGCTTGTC | |
GAPDH | F-CGGAGTCAACGGATTTGGTCGTAT |
R-AGCCTTCTCCATGGTGGTGAAGAC | |
pRL-TK-WIF-1 | F-CGGAGTCAACGGATTTGGTCGTAT |
R-AGCCTTCTCCATGGTGGTGAAGAC | |
Methylated primer | F- 5-CGTTTTATTGGGCGTATCGT-3 |
R- 5-ACTAACGCGAACGAAATACGA-3 | |
Unmethylated primer | F- 5-GGGTGTTTTATTGGGTGTATTGT-3 |
R-5-AAAAAAACTAACACAAACAAAATACAAAC-3 |