Fig. 2
From: Indirubin inhibits Wnt/β-catenin signal pathway via promoter demethylation of WIF-1
The effect of indirubin on transcriptional activity in wif-1 and β-catenin. (a) Luciferase reporter system was used to detect wif-1activity, the results showed concentration raise of pRL-TK-WIF-1-Luc dual luciferase activity after treatment by indirubin(1 μM) compared to the activity in the negative control group, inversely, the concentration reduces of pRL-TK-DNMT-1-Luc dual luciferase activity after exogenously added DNMT1 in HaCaT cells compared to the activity in the negative control group. (b) TOP Flash and FOP Flash reporters are widely used to evaluate the β-catenin-dependent wnt/β-catenin signal. Wnt/β-catenin signal activity was inhibited when treatment with indirubin(1 μM), similar results was observed when exogenously added wif-1. **P < 0.01, ***P < 0.001. (N = 3)