Fig. 3
PAMs suppressed the cells migration in human glioma TMZ-sensitive (U251S), TMZ- resistant (U251R) and mouse GL-26 cells cancer either alone or in combination with TMZ We determine the migration of glioma cells by treating with (a) PAMs alone, 1.2 × 106 cells were seed in 24 well and incubated PAMs at various concentrations (0, 50, 100, 150 μg/ml) for 24 h or (a) PAMs in combination with low dose of TMZ. Cells were treated by PAMs (50 μg/ml), TMZ (15 μM), or PAMs (50 μg/ml) + TMZ (15 μM) and then cell numbers were counted and quantified. Data represent the mean ± SEM. The p-value was calculated by t-test. (*p < 0.05, **p < 0.01, ***p < 0.001 compared to untreated control. #p < 0.05, ##p < 0.01, ###p < 0.001 compared to TMZ alone). The untreated control groups were taken as 100%. Experiments were performed in triplicate and repeated three times with similar results (n = 3)