Fig. 2

Curcumin-inhibited OCP migration was not affected by CCR1 overexpression. (a) Protein and mRNA levels of CCR1 in viruses (LV-Cont or LV-CCR1)-transduced OCPs. (b) OCPs was treated with 25 μM curcumin or PBS for 24 h, and CCL2 (10 ng/mL) was added into the supernatant of treated OCPs. The migration of OCPs exposed to the above supernatant placed in the lower chamber is observed by Transwell assays. Scale bar, 100 μm. (c) The histogram shows the number of migrated OCPs in B, *P < 0.05 by Student’s t-test; ^lettersP < 0.05 by one-way ANOVA. Data are expressed as mean ± SEM from three independent experiments. CUR, curcumin; OCP, osteoclast precursor; SUP, supernatant; SUP/OCP-PBS, supernatant of OCPs treated with PBS; SUP/OCP-CUR, supernatant of OCPs treated with curcumin