Fig. 4

Fraction F reduced NF-κB activation. a RAW-Blue cells were treated for 0.5 h with 12.5 μg/ml, 25 μg/ml, 50 μg/ml or 100 μg/ml Fraction F, followed by incubated with or without 1 μg/ml LPS for 24 h. NF-κB transcriptional activity were measured by NF-κB reporter assay. b RAW264.7 macrophages were treated with 25 μg/ml Fraction F, followed by incubated with or without 1 μg/ml LPS for 0–60 min. The phosphorylation levels of IKK-α and I-κB-α in the cell lysates were assayed by Western blot. NF-κB transcriptional activity are expressed as means ± SD of three independent experiments. Western blot results are representative of three different experiments. *P < 0.05 compared to LPS alone