Fig. 6
QLQX increased mitophagy level in hypoxic injured H9c2 cells. a: Representative photomicrographs of GFP-LC3 and red fluorescent protein (RFP)-tagged HBmTur-Mito staining in H9c2 cells. The green fluorescence dots indicate the autophagy. The red fluorescence dots indicate the mitochondria. The orange fluorescence indicates the mitophagy, 400×. b: The co-localization efficient of Mitochondria and LC3. Data are presented as mean ± SD, ** Compared to control group, P < 0.01; ## Compared to 1% O2 group, P < 0.01. n = 3 per group. c: Western blot analysis of mitophagy markers of LC3, Parkin, Pink1and p-Parkin in different groups. d: Column diagram showing the relative expression level of LC3, Pink1,Parkin and pParkin. Data are presented as mean ± SD, ** Compared to control group, P < 0.01; ## Compared to 1% O2 group, P < 0.01, # Compared to 1% O2 group, P < 0.05, n = 3 per group. The comparison of measurement data between two groups was carried out by independent-sample T test