Fig. 1

Salsola komarovii ethanol extract (SEE) as a glucocorticoid receptor (GR) ligand induces nuclear GR translocation. Binding affinity of a SEE and b hydrocortisone (HC) to the GR. Data are shown as fluorescence polarization (mP). The results are presented as mean ± standard error of the mean (SEM) of three independent experiments. c Immunofluorescence analysis of GR translocation into nucleus by HC or SEE. HaCaT cells were incubated for 24 h with 1 μM HC (positive control) or 40 μg/mL SEE followed by staining of GR (red fluorescence) and nucleus with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, blue fluorescence); original magnification 400×; scale bar = 100 μm