Fig. 2

Betulinic acid inhibit PANC-1 and SW1990 cells Colony formation and invasion. a Colony formation assay of PANC-1 and SW1990 cells incubated with betulinic acid (20 μM, 60 μM) or an equal volume of DMEM medium for 24 h. *Data are presented as mean ± SD, N = 3; *, P < 0.05;**, P < 0.01; ***, P < 0.001; ****, P < 0.0001, compared with control. b Transwell assay following PANC-1 and SW1990 cells incubated with Betulinic acid (20 μM, 60 μM) or an equal volume of DMEM medium for 24 h. *Data are presented as mean ± SD, N = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, compared with control