Fig. 6

Inhibition of autophagy enhanced apoptosis induced by myricetin in HCT116 and SW620 cells. a Cells, with or without pre-treatment with 3-MA for 1 h, were treated with 100 μmol/L myricetin for 48 h. Cell viability was investigated with a resazurin cell viability assay. b-d The Western blots show densitometry by ImageJ, and the myricetin treatment group was compared with the control group. Bax, Bcl-2, and LC3-I/II were measured in cells following treatment with 100 μmol/L myricetin for 48 h, with or without pre-treatment with 3-MA for 1 h. The experiment was repeated three times (*p < 0.05, **p < 0.01, and ***p < 0.001)