Fig. 3

N. vomica promotes nociceptive sensation after nerve injury. a Paw withdrawal latency in response to thermal stimulus in normal chow (blue columns, n=7) or N. vomica chow (red columns, n=7) groups. Measurements were obtained before and after the crush injury. Two-way repeated-measure ANOVA (diet x time) showed a significant effect of time (F_(2,26)=61.83, P<0.0001), a non-significant effect of diet (F_(1,13)=4.61, P=0.051) and a non-significant interaction between factors (F_(2,26)=1.69, P=0.203). Post-hoc pairwise comparisons with Benjamini-Hochberg correction revealed significant differences at day 2 and 8 after lesion in normal chow-fed mice and at day 2 after lesion in N. vomica chow-fed mice (^##P<0.01; ^###P<0.001). b Time to first lick in response to formalin application as in a. Two-way repeated-measure ANOVA (diet x time) showed a significant effect of time (F_(1,13)=6.01, P=0.029), a non-significant effect of diet (F_(1,13)=2.89, P=0.112) and a non-significant interaction between factors (F_(1,13)=1.19, P=0.295). Post-hoc pairwise comparisons with Benjamini-Hochberg correction revealed significant differences at 14 days after lesion in normal chow-fed mice (^#P<0.05; brackets indicate that the correction Q-value, equal to 0.061, almost attained significance)