Fig. 2
Effect of PM extract on DPCs’ protein expression related to hair cycle. DPCs (1 × 106 cells/100 mm dish) were treated with 1 μg/ml, 10 μg/ml and 100 μg/ml of PM extract for 24 h. For DP spheroids, 10 μg/ml and 20 μg/ml of PM extract were treated at the start of spheroid formation. a The protein level of DKK-1 in whole cell lysate of 2D cultured human DPCs and (b) supernatant. c Relative mRNA expression of Bcl2 and BAD in 2D cultured DPCs. d Bcl2 expression in 3D DP spheroids. e Bcl2 expression in cryo-sectioned 3D DP spheroids. Data are presented as mean ± SD. n > 3 for each group. *p < 0.05, **p < 0.01 compared with control group