Table 1 Primer sequences for RT-PCR
Gene | Accession | Sense primer sequence (5'-3') | Antisense primer sequence (5'-3') | Annealing temperature[°C] |
|---|---|---|---|---|
Rex1 | NM_009556 | ACGAGTGGCAGTTTCTTCTTGGGA | TATGACTCACTTCCAGGGGGCACT | 65 |
Nanog | AB903574 | GCTTACAAGGGTCTGCTACT | CCTCAGGACTTGAGAGCTTT | 60 |
GFP | NC_011521 | AGAAGAACGGCATCAAGGTG | CTCGTTGGGGTCTTTGCTCA | 65 |
GATA4 | NC_000080 | GCAGCAGCAGTGAAGAGATG | GCGATGTCTGAGTGACAGGA | 60 |
cTn I | NC_000073 | CTCCTCTGCCAACTACCGAG | CTCAAACTTTTTCTTGCGGC | 60 |
cTn T | NC_000067 | ATCCCCGATGGA | TTCCCACGAGTTTTGGAGAC | 65 |
Cx43 | NC_000076 | TTGACTTCAGCCTCCAAGG | AATGAACAGCACCGACAGC | 65 |
CFTR | NM_021050 | AGTTTCCTGGACAGCTCA | CTAATGGCCTGCTGGAAGAT | 60 |
CLC2 | NM_009900 | CTTAGAGTGGGAAGAACA | CTCCTTTAGGGTGACAATCC | 60 |
βactin | NM_007614 | TTCCTTCTTGGGTATGGAAT | GAGCAATGATCTTGATCTTC | 60 |