Fig. 1

Generation of cardiomyocytes from iPS cells. a: Time course for the generation of cardiomyocytes (CMs). b: Undifferentiated mouse iPS cells and iPS-CMs. Left panel: phase contrast Right panel: Nanog-promoter-driven GFP. Bars indicate 100 μm. c: RT-PCR analysis of gene expression in undifferentiated iPS cells, iPS-CMs, and the heart, using specific primers to identify undifferentiated markers, CM markers, chloride channel markers, and a ubiquitous housekeeping gene, β-actin, as indicated in the left column. d: Immunocytochemistry of iPS-CMs, FCMs, and ACMs using cTnI and Cx43 antibodies. Bars indicate 50 μm