Fig. 4

Effect of EECM on mouse primary cells isolated from BL/6 mice. a-c Real-time PCR analysis and (b) western blot analysis of osteoblastic markers in primary calvarial osteoblasts. TRAP activity in (e) mouse primary BMCs and (f) co-culture of primary calvarial osteoblasts and BMCs. *p < 0.05, **p < 0.01, ***p < 0.001, compared to differentiated cells