Fig. 11

Effects of LY294002 on the protection of SMI on DOX-induced apoptosis in H9c2 cells. Cells were pretreated with SMI (0.5%) for 24 h, LY294002 (10 μM) for 1 h, then stimulated with DOX (0.5 μM) for 24 h. a Damaged nucleus was determined by Hoechst 33342 staining. Damaged nuclei are marked with white arrows. b Cell apoptosis was determined by TUNEL assay. c Protein levels of cleaved caspase-9, caspase-9, cleaved caspase-3 and caspase-3 were assessed by Western blot. **P < 0.01 vs. Control; ^##P < 0.01 vs. DOX; ^$P < 0.05 DOX + SMI vs. DOX + SMI + LY294002; ^$$P < 0.01 DOX + SMI vs. DOX + SMI + LY294002