Fig. 4

a) Percent change in the distribution of cell cycle phases in tumor tissues of Inocul mice treated with PFT. Each value represents the mean of 8 mice/group. * P ≤ 0.01 vs. inocul control group; † P < 0.05 vs. pre-inocul group; ‡ P < 0.01 vs. pre-inocul group. b) Effect of PFT on apoptosis as detected by Annexin V/PI and analyzed by flow cytometry in EAC bearing mice. The early apoptotic cells stained with Annexin V-conjugated Alexa flour 488 dye were shown in the lower right (LR) quadrant of the histogram, whereas late apoptotic cells were shown in the upper right (UR) quadrant. Viable cells were shown in lower left (LL), while the necrotic dead cells were in the upper left (UL) quadrant of the histogram. One representative histogram is shown from 6 individual mice/group