Fig. 3

Cytological effects of GA and Myr in SKOV-3 cells. Apoptosis induction (a), morphological changes (b), and DNA content in different phases of the cell cycle (c) were determined by flow cytometry, after 24 h of treatment with GA (50 μg/mL) and Myr (166 μg/mL) and using: Annexin-V, immunofluorescence with α/β-tubulin antibody, and propidium iodide, respectively. Results show the mean ± S.D. of three biological replicates (n = 3, in triplicates); *, p ≤ 0.05 vs. the control group without treatment (0.5% DMSO in 1X PBS, ANOVA). Paclitaxel was used as a positive control at 5 μg/mL. PI, propidium iodide