Fig. 3

The inhibitory effect of R. acetosa extract on [Ca2+]i elevation and granule secretion. a Washed platelets were incubated with a calcium fluorophore (5 μM, Fura-2/AM), following treatment with different concentrations of R. acetosa extract and stimulated with collagen. b Platelets were pre-incubated with or without R. acetosa extract (25–200 μg/mL) in the presence of 1 mM CaCl₂ for 2 min at 37 °C with stirring and stimulated with collagen for 5 min. Reaction was stopped and ATP release assay was carried out. The results are presented as the mean ± SD of at least four independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 versus control