Table 2 Primers used for real-time RT-PCR
gene | Forward Primer(5′-3′) | Reverse Primer(5′-3′) |
|---|---|---|
caspase8 | TTGAACAATGAGATCCCCAAA | CCATTTCTACAAAAATTTCAAGCAG |
claudin-3 | TCTCCCAGCCTACGGAGTTA | CAGTTCCCATCTCTCGCTTC |
claudin-11 | TCACAACGTCCACCAATGACTG | GGCACATACAGGAAACCAGATG |
Fas | GCAGACATGCTGTGGATCTGG | TCACAGCCAGGAGAATCGCAG |
FasL | TCCAGGGTGGGTCTACTTACTAC | CCCTCTTACTTCTCCGTTAGGA |
GAPDH | TGTGTCCGTCGTGGATCTGA | TTGCTGTTGAAGTCGCAGGAG |
Ki67 | GCTGTCCTCAAGACAATCATCA | GGCGTTATCCCAGGAGACT |
occludin | CTTCTGCTTCATCGCTTCC | CTTGCCCTTTCCTGCTTTC |
ZO-1 | ACAAACAGCCCTACCAACC | CCATCCTCATCTTCATCTTCTTC |
ZO-2 | GTTTTTCTTCGTCCTAGTCCC | CATCCATCCCTTCCATCTTTC |