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Table 1 The specification of the primers for amplification of the targeted genes

From: Experimental validation and computational modeling of anti-influenza effects of quercetin-3-O-α-L-rhamnopyranoside from indigenous south African medicinal plant Rapanea melanophloeos

Gene namePrimer sequence (5′ to 3′)Accession numberPositionSize (bp)Tm (°C)Optimized annealing temperature (Ta) (°C)
IL-6-FGTTCGGATAATGTAGCCTNM_001003301.1633–65013540.653.9
IL-6-RTCACAGAGAACAACATAACT 751–768 40.5 
CCL-2-FGTGATCTTCAAGACCGTCCTAANM_001003297.1191–21213047.959.5
CCL-2-RTTCAGAGTGAGTATTCATGGCTT 299–321 46.6 
IFN-β-FAAACTTCACCTGGGACAANM_001135787.1390–40711840.655.9
IFN-β-RTTTCTGCTTGGACTATTGT   39.5