Fig. 3
EFSF exerted protective effect against the oxaliplatin-induced neurotoxicity. (a-c) The PC12 cells were differentiated in the DM as described in the Methods, and co-treated with oxaliplatin (LOHP, 200 nM) and the indicated concentration of EFSF or amifostine (0.5 mM) for 72 h. (a) On day 3 of the during treatment, images were photographed under an inverted microscope (× 200 magnification) and the neurites sprouting were counted. The number of neural PC12 cells extending neurites (b) and their total neurite lengths (c) were analyzed using an image software. The data from at least triplicate experiments represent the mean ± SD. *** p < 0.001 vs. vehicle. ## p < 0.01, ### p < 0.001 vs. LOHP alone. (d&e) Rat DRG cells (4 × 104) were cultured on the insert with 1 μm pores membrane and treated with LOHP (5 μM) and/or the indicated concentration of EFSF for 7 days. (d) The neurites of DRG cells were stained with Neurite Stain Solution, and the representative images were photographed under an inverted microscope (× 200 magnification). (e) The extracts of stained neurites were quantitated using a multi-plate reader at 562 nm, and neurite outgrowth was presented as a relative percentage compared to vehicle. ** p < 0.01 vs. vehicle, *** p < 0.001 vs. vehicle, ### p < 0.001 vs. LOHP alone