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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Anti-adipogenic and anti-obesity activities of purpurin in 3T3-L1 preadipocyte cells and in mice fed a high-fat diet

Fig. 5

Western Blot analysis of intracellular proteins from purpurin-treated cells in: a early-stage differentiation (2 d after MDI inducement), and b late-stage differentiation (8 d after MDI inducement). Cells were treated for the first 48 h with MDI and purpurin, after which these substances were removed from the culture. Figures represent results from at least three individual experiments. AMPK activity was expressed as the ratio phosphorylated to non-phosphorylated AMPK. Protein expression was calculated relative to β-actin, a constitutively expressed cellular protein

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