Fig. 6

AVE counteracted the inhibited effect of hair follicle growth caused by substance P in vitro. Mouse vibrissa follicles from 5-week-old male C57BL/6 mice were isolated and cultured. Each group used 10–15 follicles. a The length of vibrissa follicles was measured daily for 8 days. ●, the control group; ▲, addition of 10^− 8 mol/L SP; ■, addition of 10^− 8 mol/L SP and 10 μg/ml AVE. *p < 0.05 compared with control group; #p < 0.05 compared with SP group. b Western blot analysis was used to examine the expression of NK-1R in murine vibrissa follicles. Equal protein loadings were confirmed using β-actin antibody. Densitometric scanning of band intensities obtained from three separate experiments by the Quantity One 1D analysis software program was used to quantify the change of proteins expressions (control value taken as onefold in each case). The data showed the mean ± S.E.M of three experiments. *p < 0.05 compared with control group; ##p < 0.01 compared with SP group