Fig. 3

AS-IV reversed high glucose-induced endothelial nitric oxide synthase (eNOS) Serum NO (a) and Renal Tissue NO (b) after AS-IV treatment in rats were detected by Nitric Oxide (NO) assay kit. n = 6 per group. Results are represented as mean and SD. c representative blot image of acetylated eNOS. d Western blot analysis for the expressions of eNOS, p-eNOS (Ser1177) and p-eNOS (Thr495). ****p < 0.0001 vs. control; ##p < 0.01 vs. DN. &p < 0.05