Table 1 The primer sequences used in this study
Primers | Sequences |
|---|---|
iNOS | Forward 5′-ttgtgcatcgacctaggctggaa-3′ Reverse 5′-gacctttcgcattagcatggaagc-3′ |
COX-2 | Forward 5′-gtactggctcatgctggacga-3′ Reverse 5′-caccatacactgccaggtcagcaa-3′ |
IL-1β | Forward 5′-ggcaggcagtatcactcatt-3′ Reverse 5′-cccaaggccacaggtattt-3′ |
IL-6 | Forward 5′-gaggataccactcccaacagacc-3′ Reverse 5′-aagtgcatcatcgttgttcataca-3′ |
TNF-α | Forward 5′-tggaactggcagaagaggca-3′ Reverse 5′-tgctcctccacttggtggtt-3′ |
TRAP | Forward 5′-acttccccagcccttactaccg-3′ Reverse 5′-tcagcacatagcccacaccg-3’ |
NFATc1 | Forward 5’-tgctcctcctcctgctgctc-3′ Reverse 5′-cgtcttccacctccacgtcg-3’ |
c-Fos | Forward 5’-atgggctctcctgtcaacac-3′ Reverse 5′-ggctgccaaaataaactcca-3’ |
MMP-9 | Forward 5’-cgacttttgtggtcttcccc-3′ Reverse 5′-tgaaggtttggaatcgaccc-3’ |
CTK | Forward 5’-aggcggctatatgaccactg-3′ Reverse 5′-ccgagccaagagagcatatc-3’ |
CA2 | Forward 5’-ctctcaggacaatgcagtgctga-3′ Reverse 5′-atccaggtcacacattccagca-3’ |
OSCAR | Forward 5’-ctgctggtaacggatcagctccccaga-3′ Reverse 5′-ccaaggagccagaaccttcgaaact-3’ |
ATP6v0d2 | Forward 5’-atggggccttgcaaaagaaatctg-3′ Reverse 5′-cgacagcgtcaaacaaaggcttgta-3’ |
GAPDH | Forward 5′-ggactgtggtcatgagcccttcca-3′ Reverse 5′-actcacggcaaattcaacggcac-3′ |