Isolation of bioactive compounds from Bergenia ciliata (haw.) Sternb rhizome and their antioxidant and anticholinesterase activities

Table 3 Percent AChE and BChE inhibition potentials of Bergenia ciliata Rhizome Crude extract and their sub fractions

Sample	Concentration (μg/ml)	Percent AChE (mean ± SEM)	AChE IC₅₀ (μg/ml)	Percent BChE (mean ± SEM)	BChE IC₅₀ (μg/ml)
Crude	1000	90.22 ± 1.15 ^ns		88.22 ± 0.71**
	500	80.15 ± 0.70**	72	80.47 ± 0.86 ^ns	85
	250	71.28 ± 0.73 ^ns		67.90 ± 3.30***
	125	57.26 ± 0.42**		56.75 ± 2.25 ^ns
n-Butanol	1000	82.33 ± 1.07***		80.43 ± 1.15***
	500	70.23 ± 3.31***	140	71.80 ± 0.90***
	250	58.67 ± 1.20***		61.20 ± 0.65***	150
	125	49.93 ± 0.75***		48.83 ± 2.33***
Ethyl acetate	1000	75.32 ± 2.45***		73.26 ± 0.60***
	500	66.34 ± 0.60***		60.31 ± 0.81***
	250	53.66 ± 1.17***	185	50.33 ± 2.33***	215
	125	44.76 ± 0.73***		35.85 ± 0.68***
Chloroform	1000	66.69 ± 0.45***		69.19 ± 0.72***
	500	57.39 ± 3.26***		60.66 ± 0.94***
	250	46.19 ± 1.08***	311	45.75 ± 0.42***	350
	125	36.80 ± 0.73***		34.69 ± 0.49***
Aqueous	1000	60.45 ± 2.33***		65.37 ± 0.50***
	500	55.63 ± 1.40***		51.27 ± 0.81***
	250	43.11 ± 0.78***	400	37.63 ± 0.46***	470
	125	31.66 ± 0.76***		24.31 ± 2.42***
n-Hexane	1000	50.54 ± 1.33***		60.66 ± 0.60***
	500	45.80 ± 2.26***	650	47.56 ± 0.60***
	250	30.38 ± 0.47***		33.46 ± 0.88***	560
	125	18.66 ± 2.30***		20.38 ± 0.68***
Galanthamine	1000	95.32 ± 0.88		94.50 ± 0.71
	500	87.74 ± 0.55	45	84.66 ± 1.20	60
	250	76.44 ± 0.60		76.72 ± 0.72
	125	64.58 ± 0.54		60.83 ± 0.69

Galanthamine was used as a positive control. Data is represented as (mean ± S.E.M) n = 3. Values significantly different as compared to positive control, *: P < 0.05, **: P < 0.01, ***: P < 0.001, ns: P > 0.05

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