Fig. 4

Alterations of prefrontal cortical neurons induced by CUMS as well as XYS and Flu treatment were measured using hematoxylin-eosin staining a and Nissl staining. b Demonstrating pathologic changes and neuronal morphology of the PrL region of PFC. The micrographs in the first row were captured using a low-magnification microscope (scale bar = 100 μm, 100× magnification); the remaining micrographs were captured using a higher-magnification microscope (scale bar = 20 μm, 400× magnification). c. Quantitative analysis of the neuron numbers of Nissl-stained neurons in the PFC PrL region. The Nemenyi method is used for data comparative analysis between groups. Values are presented as the medians and interquartile ranges, (n = 5), ^**P < 0.01 versus the control group; ^#P < 0.05, ^##P < 0.01 versus the CUMS group