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Table 3 Analytical parameters of LC-MS/MS method for the analysis of 15 phytochemicals

From: Quercus infectoria gall extracts reduce quorum sensing-controlled virulence factors production and biofilm formation in Pseudomonas aeruginosa recovered from burn wounds

No. Analytes RTa Precursor ion (m/z)b R2c RSD %d Recovery (%) Ue
Intraday Interdays Intraday Interdays
1 Protocatechuic acid 7.00 153.4 0.9909 0.0060 0.0060 1.0096 0.9988 0.0215
2 Chlorogenic acid 8.03 353.3 0.9910 0.0074 0.0055 0.9941 0.9999 0.0299
3 Luteolin-7-glucoside 13.20 447.0 0.9939 0.0052 0.0037 1.0014 1.0072 0.0086
4 Rutin 13.67 609.1 0.9902 0.0063 0.0070 1.0049 1.0037 0.0136
5 Hesperidin 13.68 611.1 0.9942 0.0081 0.0073 1.0053 0.9994 0.0162
6 Hyperoside 13.69 463.0 0.9905 0.0074 0.0056 1.0039 1.0015 0.0126
7 Apigetrin 14.54 431.0 0.9902 0.0047 0.0067 1.0060 1.0047 0.0132
8 Quercitrin 14.98 447.0 0.9918 0.0079 0.0063 0.9999 1.0002 0.0133
9 Astragalin 15.13 447.0 0.9900 0.0086 0.0077 1.0002 1.0017 0.0153
10 Quercetin 17.10 301.2 0.9962 0.0177 0.0227 1.0010 1.0012 0.0573
11 Luteolin 17.78 285.2 0.9901 0.0119 0.0079 0.9961 1.0007 0.0188
12 Apigenin 19.20 269.2 0.9910 0.0087 0.0090 0.9985 1.0022 0.0181
13 Pseudohypericin 26.34 519.0 0.9908 0.0061 0.0089 1.0033 1.0034 0.0172
14 Hyperforin 28.97 535.3 0.9901 0.0218 0.0164 1.0076 1.0061 0.0418
15 Hypericin 30.18 503.0 0.9925 0.0093 0.0095 1.0104 1.0034 0.0189
  1. aRT Retention time
  2. bPrecursor ion(m/z) Molecular ions of standard compounds
  3. cR2 Correlation coefficient
  4. dRSD Relative standard deviation
  5. eU (%) Percent relative uncertainty at 95% confidence level (k = 2)