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Fig. 7 | BMC Complementary and Alternative Medicine

Fig. 7

From: Study of the antitumor mechanisms of apiole derivatives (AP-02) from Petroselinum crispum through induction of G0/G1 phase cell cycle arrest in human COLO 205 cancer cells

Fig. 7

Effect of AP-02 on expression of G0/G1 phase regulatory and metastasis-related proteins in COLO 205 xenograft tumor tissues. Tumor tissues were dissected to isolate protein lysate extracts. Protein extracts (100 μg per lane) were separated via SDS-PAGE. After electrophoresis, proteins were transferred onto Immobilon-P membranes, which were probed with the proper dilution of specific antibodies. Proteins were then detected using the NBT/BCIP system. Membranes were also probed with anti-GAPDH antibody to correct for differences in protein loading. M, molecular weight marker. Representative data from two of seven tested animals are shown. The number below each line was detected by densitometry analysis and indicates the relative intensity of protein expression compared to the control-treated group (defined as 1 in mouse #1)

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