Fig. 4

Costunolide inhibited the growth and induced mitochondria-mediated apoptosis of gastric tumor in xenografted nude mice. a Photograph of gastric tumor is taken after sacrificing mice. There are 4 groups: blank, DMSO, cisplatin (2 mg/kg) and costunolide (50 mg/kg) group. b Body weight of mice was measured every three day. c Tumor size of mice was measured by vernier caliper every three day. d Tumor weight of mice was measured after sacrificing mice. e Tunel staining of gastric tumor. f Statistical data of Tunel staining positive cells. g Apoptosis related proteins (Bax, Bcl-2, cleaved caspase 9, cleaved caspase 3, β-actin is internal reference protein) of gastric tumor of nude mice is detected by WB. h Apoptosis related genes (puma, Bax and Bcl-2, GAPDH is internal reference gene) expression of gastric tumor is detected by Q-PCR. Compared to control group, *P < 0.05, **P < 0.01. Compared to cisplatin group, ^#P < 0.05, ^##P < 0.01